Splicing to Keep Cycling: The Importance of Pre-mRNA Splicing during the Cell Cycle

Pre-mRNA splicing is a crucial process that allows proper progression of the cell cycle. Thus, inhibiting spliceosome or silencing specific factor often promotes cell-cycle arrest. Splicing factors are regulated by transcription and post-translational modifications to alter their function fit requirement each phase. changed regulating protein–protein protein–RNA interactions, intracellular localization, trafficking, activation. Chromatin emerging as novel key regulator alternative splicing. dynamics during cycle can potentially promote pattern allow progression. fundamental in mammalian gene expression, plays an extensive role generating protein diversity. Because majority genes undergo pre-mRNA splicing, most cellular processes depend on function. We focus describe its dependence accurate outline known isoforms. discuss different levels regulation such changes expression factors. effect chromatin In addition, we component SF3B1, which mutated many types cancer, link between SF3B1 inhibitors this functions at centromere ensure correct chromosome alignment segregation. short non-coding RNA molecules (50–2000 nt length) transcribed from DNA sequence enhancer regions have transcriptional regulation. enzyme removes methyl groups histones epigenetic modification. nuclear domains enriched located interchromatin nucleoplasm cells. basic structural unit packaging eukaryotes consists segment wound around eight histone proteins. Nucleosomes subunit chromatin. triple-stranded nucleic acid structure, composed DNA:RNA hybrid associated non-template single-stranded DNA, formed variety circumstances. class small (~150 found speckles Cajal bodies nucleus eukaryotic Their primary processing nucleus. also called auxiliary domains, these proline-, glycine-, acid-rich. These speculated provide specificity RNA-binding domain hnRNP family. This facilitated 3D structure fine-tunes RNA–protein interaction.